Denhardt's solution

Denhardt solution is a solution used in biochemistry. Edit Source Text

The Denhardt solution is used in a Southern blot, a Northern blot, a DNA microarray or a fluorescence in situ hybridization to block unspecific binding sites for nucleic acids on the blot membrane or the microarray or the tissue section. It is added to both blocking and hybridization solutions. To increase its viscosity, it contains Ficoll (Type 400, 0.1-0.2 g / L), as well as blocking reagents 0.1-0.2 g / L polyvinylpyrrolidone and 0.1-0.2 g / L bovine serum albumin. Because of the protein fraction by the albumin, the solution, which is applied as a concentrated stock solution, is sterile-filtered. In a Southern blot, the buffer containing the surfactant sodium lauryl sulfate is added, in addition to the Denhardt solution, heat-denatured DNA from salmon or herring sperm before use. Single-level Edit source text

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